1.Describe two variables that affect the rate of diffusion.
a. Molecular weight
b. Solute concentration
2.Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction?
3.Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction?
4.Put the following in order from smallest to largest molecular weight: glucose, sodium chloride, albumin, and urea.
Activity 2 Simulated Facilitated Diffusion
1.Explain one way in which facilitated diffusion is the same as simple diffusion and one way in which it differs.
2.The larger value obtained when more glucose carriers were present corresponds to an increase in the rate of glucose transport. Explain why the rate increased. How well did the results compare with your prediction?
3.Explain your prediction for the effect Na+Cl− might have on glucose transport. In other words, explain why you picked the choice that you did. How well did the results compare with your prediction?
Activity 3 Simulating Osmotic Pressure
1.Explain the effect that increasing the Na+Cl− concentration had on osmotic pressure and why it has this effect. How well did the results compare with your prediction? ad to increase in pressure on the right side to prevent the water from crossing the membrane.
2.Describe one way in which osmosis is similar to simple diffusion and one way in which it is different.
3.Solutes are sometimes measured in milliosmoles. Explain the statement, “Water chases milliosmoles.”
4.The conditions were 9 mM albumin in the left beaker and 10 mM glucose in the right beaker with the 200 MWCO membrane in place. Explain the results. How well did the results compare with your prediction?
Activity 4 Simulating Filtration
1.Explain in your own words why increasing the pore size increased the filtration rate. Use an analogy to support your statement. How well did the results compare with your prediction?
2.Which solute did not appear in the filtrate using any of the membranes? Explain why.
3.Why did increasing the pressure increase the filtration rate but not the concentration of solutes? How well did the results compare with your prediction?
Activity 5 Simulating Active Transport
1.Describe the significance of using 9 mM sodium chloride inside the cell and 6 mM potassium chloride outside the cell, instead of other concentration ratios.
2.Explain why there was no sodium transport even though ATP was present. How well did the results compare with your prediction?
3.Explain why the addition of glucose carriers had no effect on sodium or potassium transport. How well did the results compare with your prediction?
4.Do you think glucose is being actively transported or transported by facilitated diffusion in this experiment? Explain your answer.
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